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Sources Of Error In Gel Filtration Chromatography

You can only upload files of type PNG, JPG, or JPEG. You can only upload a photo (png, jpg, jpeg) or a video (3gp, 3gpp, mp4, mov, avi, mpg, mpeg, rm). It will interact with the column at this point but will not interact when the Tris is used. Cory · 6 years ago 0 Thumbs up 0 Thumbs down Comment Add a comment Submit · just now Report Abuse Add your answer Where are errors most likely to occur click site

Title: Affinity Chromatography Obje Experiment 6 2 pages Experiment 2 BSA Baylor BIO 4141 - Spring 2013 Erik Odom 02/11/2012 4141-A Experiment 1: Determination of the Extinction Coefficient Experiment 2 BSA Difference between natural and artificial media in animal tissue culture? What are centimeters? References: • G.E.

Title: Affinity Chromatography Obje Experiment 6 Erik, Odom Page5 / 6 The sources of error in this experiment could have come... Sign up to view the full document. Sign up to access the rest of the document. Sign up to view the full version.

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More questions What situation has to occur for you to say that person needs to be whacked up side the head with a 2 by 4? You can only upload videos smaller than 600MB. Health Sciences “Ion Exchange Chromatography, Principles, and Methods” Handbook. • Sigma catalog information on CM-sepharose and the components of the mixture (BSA and cytochrome c). • Technical Information for CM-sepharose from Follow 1 answer 1 Report Abuse Are you sure you want to delete this answer?

Would you be able to separate catalase and haemoglobin by the technique of gel-filtration? You can only upload files of type 3GP, 3GPP, MP4, MOV, AVI, MPG, MPEG, or RM. ⌂HomeMailSearchNewsSportsFinanceCelebrityWeatherAnswersFlickrMobileMore⋁PoliticsMoviesMusicTVGroupsStyleBeautyTechShoppingInstall the new Firefox» Yahoo Answers 👤 Sign in ✉ Mail ⚙ Help Account Info Help Suggestions Send Feedback Answers Home All Categories Arts & Humanities Beauty & Style Business & Course Hero is not sponsored or endorsed by any college or university.

If you over pack the column, it can damage the pores in the beads, and that clearly causes problems. Leaving solvent at the top of the column immediately before adding the solution to be filtered. Cytochrome C has a much higher pI value than BSA and it stay (+) charged when the phosphate buffer is being used. If there is already solution on top of the beads, and you have not allowed it to drain so that the top of the beads are exposed to air, the solution

This preview shows document pages 5 - 6. View Full Document Company About Us Scholarships Sitemap Standardized Tests Get Course Hero iOS Android Educators Careers Our Team Jobs Internship Help Contact Us FAQ Feedback Legal Copyright Policy Honor Code How would I use the scientific method for an experiment of how temperature affects plant growth? Ion Exchange: This preview has intentionally blurred sections.

TERM Spring '13 PROFESSOR Jonklaas Click to edit the document details Share this link with a friend: Copied! http://phabletkeyboards.com/sources-of/sources-of-error-in-liquid-chromatography.php View Full Document The sources of error in this experiment could have come from incorrectly labeling the 2 mL mark on the test tubes, not cleaning the glass cuvette well, disturbing Ask a homework question - tutors are online Because of the charge on BSA it could not interact with the column, or stick to it, and therefore eluted when this buffer was being used.

You can only upload a photo or a video. If you under pack the column, there is not enough bead surface area to interact with all of the smaller particles. The phosphate buffer’s pH was 7.00. navigate to this website The 2M Tris, at a pH of 8.00, acting as a salt, caused the cytochrome C to not interact and therefore elute while the BSA stayed in the column (or if

Answer Questions 1. Please upload a file larger than 100x100 pixels We are experiencing some problems, please try again. Both result in smaller particles moving too quickly through the column. 2.

Report this document Report View Full Document Most Popular Documents for BIO 4141 2 pages Calcium complex Baylor BIO 4141 - Spring 2013 Erik Odom CHE 2216 11/15/2012 Complexometric Determination of

Why tubulin heterodimer has a GTP bindingsite While actin has an ATP binding site? You can only upload photos smaller than 5 MB. What does "A disk read error occurred" means? Because BSA’s pI is low (4.9) it has a (-) charge for pH’s above its pI.

Over or under packing the column. Hopefully those make sense Source(s): Factors affecting filtration: http://en.wikipedia.org/wiki/Size-exclus... Yes No Sorry, something has gone wrong. http://phabletkeyboards.com/sources-of/sources-of-error-in-liquid-chromatography-lab.php View Full Document The separation of BSA from cytochrome C is possible due to a difference in the pI for each of the different compounds.

Log in Sign up Home Baylor BIO BIO 4141 Experiment write-up #2 The sources of error in this experiment could have SCHOOL Baylor COURSE TITLE BIO 4141 TYPE Notes UPLOADED BY Trending Could humans evolve into zebras? 14 answers How do we know science is right? 31 answers It's been many years since Darwin claimed Evolution is real but we still haven't Video should be smaller than 600mb/5 minutes Photo should be smaller than 5mb Video should be smaller than 600mb/5 minutesPhoto should be smaller than 5mb Related Questions Where are errors most Course Hero, Inc.

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The sources of error in this experiment could have come from incorrectly labeling the 2 mL mark on the test tubes, not cleaning the glass cuvette well, disturbing the column, or Trending Now Anna Kendrick Amber Tamblyn Jasmine Tookes Star Wars 2016 Crossovers Auto Insurance Quotes Pablo Escobar Gary Johnson Dating Sites Karolina Pliskova Answers Best Answer: The two places I have GEL FILTRATION CHROMATOGRAPHY?