Possible sources of error in coefficient of linear expansion Lab? I guess an analogy of it would be someone whose heart has stopped beating (the ice bath). ARA is the L-arabinose operon for E.coli and you must know this to understand your lab. When you put it into the water bath, the membrane undergoes heat shock so the previously made holes will seal. navigate to this website
I'm sure you're going to do this or your teacher will explain it but one way is to use CaCl2. We'd be delighted to hear from you! Now, for the sources of error part, I couldn't think of anything....could some one please suggest me some??? What you need.
Tweezers. Sources of error depend on the exact method of synthesis. There was no arabinose in the LB/Amp + plate-- only in the LB/Amp/Ara + plate. Use sterile cotton swabs.
The system returned: (22) Invalid argument The remote host or network may be down. Measure and compare the size of the kill zone to determine effectiveness of each antibacterial agent. AMP is for adenosine monophosphate, which will become cyclic AMP (cAMP). The bacteria grew into a lawn because there was no ampicillin that would hinder its reproduction.
Your cache administrator is webmaster. But I'll copy some of it to help? NC-0993 Filter Paper, 7cm, coarse. Compare your sample to known samples of bacteria, investigating where these known samples are likely to be found. 4.
As for the same dish, instead with the pGLO-induced bacteria, the bacteria grew in colonies because not all of the bacteria were able to take in the plasmid. cAMP is used for tons of things, such as forming ATP, which can later be broken into ADP + Pi (inorganic phosphate) to release energy. Thanks, but I have a question. Heat the inoculating loop until it glows red.
It was seen that the bacteria without the plasmid produced a lawn in normal conditions, but grew only in colonies in the Ampicillin condition. Or, you can press a variety of common objects like coins, combs, etc. Petri Dish Image adapted from WP Clipart Petri Dish 2 Photos from Bacteria Lab 2011-2012 Science Company Home About Us Customer Service Contact Us Login View Cart Chemicals Lab It will burn!
It will burn! http://phabletkeyboards.com/sources-of/sources-of-human-error.php Wax pencil for labeling dishes. The bacteria then reproduce and the plasmid is replicated in the process as well. Wash the excess stain off the slide by picking the slide up and holding it at an angle over the sink.
Title (Something clever) 2. More questions Possible Sources of Error in a lab experiment? Place a drop of methylene blue on the slide over where the “smudge” of bacteria and water is 7. my review here All in all, it can be concluded that bacterial transformation can affect the fitness of bacteria in different situations.
M-2149 12 x 75mm Test Tube. Inoculate each dish by streaking a pattern gently across the entire agar surface without tearing into it. You can only upload files of type PNG, JPG, or JPEG.
Bacteriology – Lab Report Guidelines Must be TYPED. You can only upload a photo (png, jpg, jpeg) or a video (3gp, 3gpp, mp4, mov, avi, mpg, mpeg, rm). Examine the slide under the microscope using the high power objective. E.coli lives inside you and this temperature is the optimal temperature for it to grow, so when you introduce each sample to each platelet of LB, AMP and ARA, you want
Before it cools (and solidifies) it can be poured into petri dishes. The other two conditions included the integration of the plasmid in the bacteria cells, and consisted of a dish with Ampicillin and one with Ampicillin and arabinose sugar. Ask in this forum if you need help with coursework, applications, and more. http://phabletkeyboards.com/sources-of/sources-of-error-with-spectrophotometry.php When you rupture the membrane, the contents inside the cell may leak so the ice bath prevents this.
Add 5 drops of crystal violet over the fixed culture. Can you make any conclusions about what locations had the most bacteria? Store upside down. Observe Known Bacteria samples Use internet sources, slide viewers, and prepared slides to identify the different shapes and types of bacteria.
Replace cover on dish, tape closed, and label each dish so you know the source of the bacteria. ARA "turns on" because it has a promoter region as well as being an operon, and as you know the promoter region (called araI for ARA) is where transcription will occur. Or using the same tool to collect different samples and thereby mixing samples that were supposed to be different? If you've learned of the mitochondria, then you'll know what ATP is for.
Education and Careers Work of any kind can get stressful at times. Terms | Legal | Privacy | Conduct Powered by vBulletin.Copyright ©2000-2016, Jelsoft Enterprises Ltd. Furthermore, it was observed how this plasmid contributed to the growth and proliferation of bacteria in different environments. Quote: Abstract: This lab focused on implementing a pre-constructed plasmid containing beta lactamase, GFP, and an arabinose operon into an E.Coli cell.
Another common technique is to divide each plate into four quadrants by marking the lid with a cross. D: Touch your finger gently to this circle. For your experiment, you have to get each of the variables (LB, AMP and ARA) inside the cell, so one way is to create small holes in the membrane using chemicals. Blood agar or tryptic soy agar with 5% sheep's blood is an excellent medium for supplying bacteria with nutrients and an environment in which we can see them grow.
Hole punch. Let us know what you tested and how your experiment turned out. LB is a lysogeny broth and it's used for the lsyogenic cycle as a way for viral reproduction. The contaminants may also stop the desired reaction from occurring entirely.
Can you make any conclusions about what objects had the most bacteria?